ᠠᠮᠢᠳᠤ ᠪᠣᠳᠠᠰ ᠰᠣᠩᠭᠤᠨ ᠰᠤᠷᠬᠤ ᠨᠢᠭᠡ -- ᠲᠤᠰᠭᠠᠢ ᠰᠡᠳᠤᠪ 5.1DNA ᠶᠢ ᠪᠦᠳᠦᠬᠦᠪᠴᠢᠯᠡᠨ ᠭᠠᠷᠭᠠᠨ ᠠᠪᠬᠤ ᠪᠠ ᠰᠢᠯᠭᠠᠨ ᠲᠤᠭᠳᠠᠭᠠᠬᠤ .pptx
DNA 5:, 1 : DNA ,DNA ᠬᠠᠮᠢᠭᠠ ᠤᠷᠤᠰᠢᠬᠤ ᠪᠤᠢ︖ DNA ᠢ᠋ᠢᠨ ᠭᠣᠣᠯ ᠠᠴᠢᠶᠠᠯᠠᠭᠴᠢ ᠨᠢ ᠶᠠᠭᠤ ᠪᠤᠢ︖ DNA ᠪᠠ ᠤᠭᠤᠷᠠᠭ ᠨᠢ ᠶᠠᠮᠠᠷ ᠢᠯᠭᠠᠭᠠ ᠲᠠᠢ ᠪᠤᠢ︖ DNA ᠢ᠋ᠢᠨ ᠲᠤᠬᠠᠢ ᠮᠡᠳᠡᠯᠭᠡ ᠵᠢ ᠡᠮᠬᠢᠳᠬᠡᠵᠤ ᠦᠵᠡ᠃,ᠦᠨᠳᠦᠰᠦᠨ ᠮᠡᠳᠡᠯᠭᠡ1,DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠬᠠᠢ ᠴᠢᠨᠠᠷ,,NaCl ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠤᠨ ᠦᠳᠬᠡᠴᠠ ᠨᠢ 0.14 mol/L ᠦᠶᠠᠰ᠂ DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠴᠠ ᠨᠢ ᠬᠠᠮᠤᠭ ᠪᠠᠭᠠ,NaCl ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠤᠨ ᠦᠳᠬᠡᠴᠠ ᠨᠢ 0.14 mol/Lᠡᠴᠠ ᠪᠠᠭᠠ ᠦᠶᠠᠰ᠂ DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠴᠠ ᠨᠢ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠤᠨ ᠦᠳᠬᠡᠴᠠ ᠨᠡᠮᠡᠭᠳᠡᠬᠦ ᠵᠢ ᠲᠠᠭᠠᠯᠳᠦᠨ ᠪᠠᠭᠠᠰᠤᠨᠠ,NaCl ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠤᠨ ᠦᠳᠬᠡᠴᠠ ᠨᠢ 0.14 mol/Lᠡᠴᠠ ᠶᠡᠬᠡ ᠦᠶᠠᠰ᠂ DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠴᠠ ᠨᠢ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠤᠨ ᠦᠳᠬᠡᠴᠠ ᠨᠡᠮᠡᠭᠳᠡᠬᠦ ᠵᠢ ᠲᠠᠭᠠᠯᠳᠦᠨ ᠨᠡᠮᠡᠭᠳᠡᠨᠡ,ᠦᠨᠳᠦᠰᠦᠨ ᠮᠡᠳᠡᠯᠭᠡ1,DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠬᠠᠢ ᠴᠢᠨᠠᠷ,0.14 mol/L ᠦᠶᠠᠰ᠂ DNA ᠨᠢ ᠢᠯᠭᠠᠷᠠᠨ ᠭᠠᠷᠤᠨᠠ 2 mol/L ᠦᠶᠠᠰ᠂ DNA ᠨᠢ ᠪᠦᠷᠢᠮᠦᠰᠦᠨ ᠠᠭᠤᠰᠤᠨᠠ,ᠰᠫᠢᠷᠲ ᠤᠨ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠳᠤ ᠠᠭᠤᠰᠬᠤ ᠴᠢᠨ᠋ᠠᠷ ᠄ ᠵᠠᠷᠢᠮ ᠬᠤᠯᠢᠴᠠ ᠠᠭᠤᠰᠤᠨᠠ ᠂ DNA ᠠᠭᠤᠰᠬᠤ ᠦᠭᠡᠢ,ᠦᠨᠳᠦᠰᠦᠨ ᠮᠡᠳᠡᠯᠭᠡ2,ᠢᠰᠭᠡᠭᠦᠷ᠂ ᠥᠨᠳᠦᠷ ᠲᠤᠯᠠᠭᠠᠴᠠ᠂ ᠤᠭᠢᠶᠠᠯᠭᠠ ᠶᠢᠨ ᠡᠳ᠋ ᠲᠦ ᠲᠠᠭᠠᠭᠠᠬᠤ ᠴᠢᠨᠠᠷ,ᠤᠭᠤᠷᠠᠭ ᠤᠨ ᠢᠰᠭᠡᠭᠦᠷ᠄ ᠢᠰᠭᠡᠭᠦᠷ ᠨᠢ ᠲᠤᠰᠭᠠᠢᠵᠢᠭᠰᠠᠨ ᠴᠢᠨᠠᠷ ᠲᠠᠢ᠂ ᠵᠥᠪᠬᠡᠨ ᠤᠭᠤᠷᠠᠭ ᠤᠨ ᠮᠣᠯᠧᠺᠦ᠋ᠯ ᠢ᠋ ᠵᠠᠳᠠᠷᠠᠭᠤᠯᠵᠤ᠂ DNA ᠳ᠋ᠦ ᠨᠥᠯᠦᠭᠡ ᠦᠭᠡᠢ ᠦᠨᠳ᠋ᠦᠷ ᠲᠤᠯᠠᠭᠠᠴᠠ ᠶᠢ ᠲᠠᠭᠠᠭᠠᠬᠤ ᠴᠢᠨ᠋ᠠᠷ ᠄ ᠤᠯᠠᠩᠬᠢ ᠤᠭᠤᠷᠠᠭ 60~80℃ ᠤᠨ ᠦᠨᠳ᠋ᠦᠷ ᠲᠤᠯᠠᠭᠠᠴᠠ ᠳᠤ ᠴᠢᠨ᠋ᠠᠷ ᠬᠤᠪᠢᠷᠠᠨᠠ ᠂ DNA ᠬᠠᠷᠢᠨ 80 ℃ ᠡᠴᠠ ᠲᠡᠭᠡᠭ᠍ᠰᠢ ᠲᠤᠯᠠᠭᠠᠴᠠ ᠳᠤ ᠰᠠᠶᠢ ᠴᠢᠨ᠋ᠠᠷ ᠬᠤᠪᠢᠷᠠᠨᠠ ᠤᠬᠢᠶᠠᠯᠭᠠ ᠵᠢᠨ ᠡᠳ᠋ ᠢ ᠲᠠᠭᠠᠭᠠᠬᠤ ᠴᠢᠨ᠋ᠠᠷ ᠄ ᠡᠰ ᠤᠨ ᠪᠦᠷᠬᠦᠬᠦᠯ ᠢ ᠵᠠᠳ᠋ᠠᠷᠠᠭᠤᠯᠵᠤ ᠴᠢᠳ᠋ᠠᠨᠠ᠂ DNA ᠳᠤ ᠨᠦ᠋ᠯᠦᠭᠡ ᠦᠭᠡᠢ,ᠦᠨᠳᠦᠰᠦᠨ ᠮᠡᠳᠡᠯᠭᠡ 3,DNA ᠵᠢ ᠰᠢᠯᠭᠠᠨ ᠬᠡᠷᠡᠴᠢᠯᠡᠬᠦ,ᠤᠷᠪᠠᠯᠵᠢ᠄ ᠬᠤᠶᠠᠷ ᠹᠸᠨ᠋ᠢᠯᠳ᠋ᠦ ᠠᠮᠢᠨ ᠨᠥᠬᠦᠴᠡᠯ᠄ ᠤᠰᠤᠨ ᠬᠠᠯᠠᠭᠠᠭᠤᠷ ᠦᠵᠡᠭᠳᠡᠯ᠄ ᠬᠦᠭᠡ ᠦᠩᠭᠡ,ᠦᠨᠳᠦᠰᠦᠨ ᠮᠡᠳᠡᠯᠭᠡ 4,ᠲᠤᠷᠰᠢᠯᠳᠠ ᠶᠢᠨ ᠳᠦᠰᠦᠪ,ᠮᠠᠲ᠋ᠧᠷᠢᠶᠠᠯ ᠰᠤᠩᠭᠤᠬᠤ ᠡᠰ ᠢ ᠬᠡᠮᠭᠡᠯᠡᠬᠦ ᠬᠣᠯᠢᠴᠠ ᠵᠢ ᠠᠷᠢᠯᠭᠠᠬᠤ ᠢᠯᠭᠠᠷᠠᠭᠤᠯᠤᠨ ᠭᠠᠷᠭᠠᠬᠤ ᠰᠢᠯᠭᠠᠨ ᠲᠤᠭᠳᠠᠭᠠᠬᠤ,ᠵᠠᠷᠴᠢᠮ ᠄ DNAᠠᠭᠤᠯᠤᠭᠰᠠᠨ ᠠᠮᠢᠳᠤ ᠪᠣᠳᠠᠰ ᠤᠨ ᠮᠠᠲ᠋ᠧᠷᠢᠶᠠᠯ ᠢ ᠴᠥᠮ ᠪᠣᠳᠣᠯᠬᠢᠯᠠᠵᠤ ᠪᠣᠯᠤᠨᠠ ᠂ ᠭᠡᠪᠡᠴᠦ DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠯᠤᠭᠳᠠᠴᠠ ᠪᠠᠷ ᠬᠠᠷᠢᠴᠠᠩᠭᠤᠶ ᠥᠨᠳᠥᠷ ᠠᠮᠢᠳᠤ ᠪᠣᠳᠠᠰ ᠤᠨ ᠨᠡᠬᠡᠳᠡᠰᠦ ᠶᠢ ᠰᠣᠩᠭᠣᠨ ᠬᠡᠷᠡᠭᠯᠡᠪᠡᠯ ᠠᠮᠵᠢᠯᠲᠠ ᠤᠯᠬᠤ ᠪᠣᠯᠤᠯᠴᠠᠭᠠ ᠨᠡᠩ ᠶᠡᠬᠡ ᠪᠣᠯᠤᠨᠠ ᠮᠠᠲ᠋ᠧᠷᠢᠶᠠᠯ᠄ ᠵᠢᠭᠠᠰᠤᠨ ᠤ ᠥᠨᠳᠡᠭᠡ᠂ ᠦᠬᠡᠷ ᠦᠨ ᠴᠢᠰᠤ᠂ ᠭᠠᠬᠠᠶ ᠶᠢᠨ ᠡᠯᠢᠭᠡ᠂ ᠨᠣᠭᠣᠭᠠ ᠶᠢᠨ ᠴᠡᠴᠡᠭ᠂ ᠪᠠᠨᠠᠨᠠ᠂ ᠲᠠᠬᠢᠶᠠᠨ ᠴᠢᠰᠤ᠂ ᠪᠥᠭᠡᠷᠡᠩᠬᠡᠶ ᠰᠣᠩᠭᠢᠨᠠ᠂ ᠬᠥᠬᠥᠲᠡᠨ ᠦ ᠪᠣᠯᠪᠠᠰᠤᠷᠠᠭᠰᠠᠨ ᠤᠯᠠᠭᠠᠨ ᠡᠰ᠂ ᠲᠣᠰᠢᠯᠣᠭ᠂ ᠸᠠᠨᠳᠧᠦ᠂ ᠥᠷᠭᠡᠰᠦᠲᠦ ᠨᠣᠭᠣᠭᠠ᠂ ᠰᠢᠩᠭᠡᠨ ᠪᠡᠶᠡᠲᠦ ᠲᠡᠵᠢᠭᠡᠯ ᠳᠣᠲᠣᠷᠠ ᠪᠣᠶᠢᠵᠢᠭᠤᠯᠤᠭᠰᠠᠨ ᠪᠦᠳᠦᠭᠦᠨ ᠭᠡᠳᠡᠰᠦᠨ ᠦ ᠰᠠᠪᠬᠠᠨᠴᠢᠷ ᠪᠠᠺᠲ᠋ᠧᠷᠢ,,,,ᠲᠠᠬᠢᠶᠠᠨ ᠴᠢᠰᠦᠨ ᠳᠦDNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠯᠤᠭᠳᠠᠴᠠ ᠡᠯᠪᠡᠭ᠂ ᠮᠠᠲ᠋ᠧᠷᠢᠶᠠᠯ ᠢ ᠤᠯᠬᠤ ᠳ᠋ᠤ ᠳᠥᠭᠦᠮ᠂ ᠠᠮᠠᠷᠬᠠᠨ ᠬᠠᠭᠠᠷᠠᠨᠠ᠃ (ᠨᠢᠮᠪᠡᠭᠡ ᠬᠦᠴᠢᠯ ᠦᠨ ᠨᠠᠲ᠋ᠷᠢ ),ᠡᠰ ᠢ ᠬᠡᠮᠭᠡᠯᠡᠬᠦ(ᠠᠮᠢᠳᠠᠨ ᠤ ᠡᠰ ᠦᠨ ᠮᠠᠲ᠋ᠧᠷᠢᠶᠠᠯ),ᠨᠡᠷᠡᠮᠡᠯ ᠤᠰᠤ ᠨᠡᠮᠡᠶᠡ ᠃ ᠨᠡᠷᠡᠮᠡᠯ ᠤᠰᠤ ᠨᠢ ᠲᠠᠬᠢᠶᠠᠨ ᠤ ᠴᠢᠰᠤᠨ ᠤ ᠡᠰ ᠲᠦ ᠠᠪᠴᠤ ᠬᠡᠯᠡᠪᠡᠯ ᠨᠢᠭᠡ ᠵᠦᠢᠯ ᠦᠨ ᠳᠣᠣᠷᠠ ᠨᠡᠪᠴᠢᠯᠲᠡ ᠢ᠋ᠢᠨ ᠰᠢᠩᠭᠡᠨ ᠪᠡᠶᠡᠲᠦ᠂ ᠤᠰᠤ ᠨᠢ ᠶᠡᠬᠡ ᠬᠡᠮᠵᠢᠶᠡ ᠪᠡᠷ ᠴᠢᠰᠤᠨ ᠤ ᠡᠰ ᠲᠦ ᠣᠷᠣᠵᠤ᠂ ᠴᠢᠰᠤᠨ ᠤ ᠡᠰ ᠢ ᠲᠡᠯᠡᠵᠦ ᠬᠠᠭᠠᠷᠠᠭᠤᠯᠤᠨᠠ᠂ ᠳᠡᠭᠡᠷᠡ ᠨᠢ ᠪᠠᠰᠠ ᠬᠤᠳᠬᠤᠨᠠ᠃,,ᠤᠬᠢᠶᠠᠯᠭᠠ ᠵᠢᠨ ᠡᠳ᠋ ᠨᠢ ᠡᠰ ᠦᠨ ᠪᠦᠷᠬᠦᠭᠦᠯ ᠢ ᠵᠠᠳᠠᠷᠠᠭᠤᠯᠵᠤ᠂ DNA ᠢ᠋ᠢᠨ ᠢᠯᠭᠠᠷᠠᠭᠤᠯᠬᠤ ᠳᠤ ᠠᠰᠢᠭᠲᠠᠶ; ᠢᠳᠡᠰᠢᠨ ᠦ ᠳᠠᠪᠤᠰᠤᠨ ᠤ ᠭᠣᠣᠯ ᠪᠦᠷᠢᠯᠳᠦᠬᠦᠨ ᠨᠢ NaCl ᠪᠠᠢᠵᠤ᠂ DNA ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠬᠤ ᠳᠤ ᠠᠰᠢᠭᠲᠠᠶ᠃,ᠬᠣᠭᠤᠯᠠᠨ ᠲᠠᠪᠤᠰᠤ ᠪᠠ ᠤᠬᠢᠶᠠᠯᠭᠠ ᠵᠢᠨ ᠡᠳ᠋ ᠨᠡᠮᠡᠨᠡ᠃,ᠡᠰ ᠢ ᠬᠡᠮᠭᠡᠯᠡᠬᠦ(ᠤᠷᠭᠤᠮᠠᠯ ᠤᠨ ᠡᠰ ᠦᠨ ᠮᠠᠲ᠋ᠧᠷᠢᠶᠠᠯ),ᠬᠣᠯᠢᠴᠠ ᠵᠢ ᠠᠷᠢᠯᠭᠠᠬᠤ,10~15min 60~75℃ ,,,ᠢᠯᠭᠠᠷᠠᠭᠤᠯᠤᠨ ᠭᠠᠷᠭᠠᠬᠤ,ᠠᠯᠭᠤᠷ ᠤᠳᠠᠭᠠᠨ ᠢ᠋ᠢᠠᠷ ᠰᠢᠯ ᠰᠠᠪᠠᠭᠠ ᠪᠠᠷ ᠨᠢᠭᠡᠨ ᠴᠢᠭᠯᠡᠯ ᠢᠶᠡᠷ ᠬᠤᠳᠭᠤᠨ ᠬᠥᠳᠡᠯᠭᠡᠨᠡ᠂ DNA ᠢ᠋ᠢᠨ ᠮᠣᠯᠧᠺᠦ᠋ᠯ ᠲᠠᠰᠤᠷᠠᠬᠤ ᠡᠴᠠ ᠰᠡᠷᠭᠡᠢᠯᠡᠨᠡ᠃,2 mol/L NaC ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠦᠶᠠᠰ᠂ DNA ᠨᠢ ᠪᠦᠷᠢᠮᠦᠰᠦᠨ ᠠᠭᠤᠰᠤᠨᠠ,ᠠᠳᠠᠯᠢ ᠬᠡᠮᠵᠢᠶᠠᠨ ᠤ ᠬᠦᠷᠭᠡᠭᠰᠡᠨ ᠰᠫᠢᠷᠲ,ᠰᠢᠯᠭᠠᠨ ᠲᠤᠭᠳᠠᠭᠠᠬᠤ,DNA + ᠬᠤᠶᠠᠷ ᠪᠸᠨᠢᠯᠳᠦ ᠠᠮᠢᠨ = ᠬᠥᠬᠡ ᠥᠩᠭᠡ,DNA ᠢ᠋ᠢᠨ ᠮᠣᠯᠧᠺᠦ᠋ᠯ ᠢ᠋ 2 mol/L NaC ᠢ᠋ᠢᠨ ᠠᠭᠤᠰᠤᠮᠠᠯ ᠳ᠋ᠦ ᠪᠦᠷᠢᠮᠦᠰᠦᠨ ᠠᠭᠤᠰᠤᠨᠠ,ᠤᠰᠤᠨ ᠬᠠᠯᠠᠭᠠᠭᠤᠷ 5min,ᠬᠠᠷᠢᠴᠠᠭᠤᠯᠬᠤ ᠲᠤᠭᠤᠢᠯᠠᠩ,
ᠮᠣᠩᠭᠣᠯ ᠳ᠋ᠣᠺ ᠤᠨ ᠬᠥᠮᠥᠷᠭᠡ